Effects of Vitamin C Supplementation to Bovine Peripheral Blood Mononuclear Cells on Antioxidant and Inflammatory Biomarker Status and Cell Viability with or without Lipopolysaccharide Stimulation

This study aimed to determine the effects of vitamin C supplementation on antioxidant and inflammatory status cell viability bovine peripheral blood mononuclear cells. Peripheral cells (PBMCs) were isolated from eighteen clinically healthy Japanese Black calves in this study. PBMCs cultured with (vitamin group) without (control stimulated or lipopolysaccharide (LPS). As a result, total capacities, which are reducing power components Fe3+ Fe 2+, culture supernatant LPS stimulation significantly higher group than those control (P < 0.05, P 0.01, respectively). Tumor necrosis factor-alpha was that 0.05). The after 72 h These results suggested is related anti-inflammatory properties obtained calves.